摘要
本文报道了用胭脂碱型致瘤农杆菌感染单子叶植物石蒜和金针菜的实验结果。石蒜和金针菜的花茎幼嫩部位被致瘤农杆菌A_(208)感染后,一个月内形成小瘤。纸电泳结果表明,所有瘤组织都含有胭脂碱,证明石蒜和金针菜的细胞可被转化并表达nos基因;本文讨论了致瘤农杆菌转化单子叶植物的范围和筛选方法。
Agrobacterium tumefaciens-mediated transformation of monocots Hemerocallis citrina and Lycoris radiata is reported here.Swellings formed on Young floral stems of H.citrina and of L.radiata within one month after infection.Expression of nos gene was confirmed by nopaline detection.Host range of A.tumefaciens in monocotyledoneae and host screening methods are discussed.
关键词
Ti质粒基因
石蒜
金针菜
植物转化
Plant transformation,Agrohacterium tumefaciens/Ti-plasmid,Hemerocallis citrina,Lycoris radiata
