摘要
用庆大霉素产生菌——棘孢小单孢菌Micromonospora echinospora 814(Gm^r,Km^r)和链霉素产生菌Streptomyces griseus No. 45(Sm^r,Lm^r)进行了原生质体融合。以抗性为选择标记,选出了融合体。其融合频率在10^(-3)—10^(-4)之间。在电镜条件下,观察了原生质体融合的详细过程,测定了融合体的产抗生素能力,其中一株融合体F106的抗生素产量比亲本菌株814高58％。用羧甲基纤维素薄层对发酵液层析表明,有一个融合体的发酵液比亲本菌株814多一个组份,但没有测出其生物活性。
Protoplast fusion was performed between gentamycin producer Micromonospora echlnospora 814 (Gm',Km') and streptomycin producer Streptomyces griseus No.45 (Smr,Lmr).Fusion bodies were selected by the resistant markers with frequencies of 10-22 to 10-4.The detailed fusion process of cytoplasm and nucleoplasm was clearly observed under EM.The resistant markers of fusion bodies were analysed.The antibiotic productivities of fusion bodies were determined,and the yield of one fusion body was increased by 58% as compared with that of parent strain 814.Thin-layer silica gel G sodiuum carboxymethyl cellulose chromatography showed that the culture broth of some fusion bodies had an unknown spot below gentamycin Cla as compared with parent 814.
关键词
棘孢小单孢菌
链霉菌
原生质体融合
Micromonospora echinospora,Streptomyces griseus,Protoplast fusion
