摘要
目的：研究幽门螺杆菌（Ｈｅｌｉｃｏｂａｃｔｅｒｐｙｌｏｒｉ，Ｈｐ）感染的一种无创伤性血清免疫荧光抗体检查技术，作为消化性溃疡（ＰＵ），慢性胃炎（ＣＧ）以及非溃疡性消化不良（ＮＵＤ）患者Ｈｐ感染的快速诊断和Ｈｐ清除与否的指标，并和内镜活检尿素酶法，直接细菌染色法比较，以确定Ｈｐ血清免疫荧光抗体的诊断价值．方法：观察对象为内镜诊断的ＰＵ２８例；ＣＧ１２２例及正常人３０例，共１８０例，均经尿素酶及Ｈｐ染色两法测试．两法均阳性者１００例（ＰＵ１７，ＣＧ８３），阴性５０例，还有阴性健康者３０例．结果：免疫荧光法测试结果，在前两法阳性组的阳性率为９６％（９６／１００），阴性组的阳性率为２２％（１１／５０），阴性健康组的阳性率为３％（１／３０）．结论：Ｈｐ血清免疫荧光与非免疫荧光比较，前者对Ｈｐ诊断特异性强，敏感性高，在粘膜内Ｈｐ定位清晰．
Objective:To develop an indirect immunofluorescence method in detection of serum anti-Heli-cobacter pylori(Hp)antibody as a rapid diagnostic method of Hp infection and a criterion of Hp clearance inpatients with peptic ulcer(PU),chronic gastritis(CG)and nonulcer dyspepsia(NUD).The authors com-pared this method with one-minute urease test(1 min UT)by endoscopy and tissue smear for Hp by Gram’sstain to determine its diagnostic value. Methods:From March 1990 to March 1994,180 cases were studied,including PU 28,CG 122,and normal subjects 30.All of them were diagnosed by endoscopy and tested by 1min UT and itssue smear examination.Among all subjects tested by the above two methods,100(PU 17,CG 83)cases were Hp positive,50 patients(PU 11,CG 39)and 30 normal subjects were Hp negative. Re-sults:By using immunofluorescence method, Hp positivity rates were 96%(96/100)in 100 Hp positive casementioned above,22%(11/50)in Hp negative patients and 3%(1/30)in 30 normal subjects. Conclusion:Comparing the indirect immunofluorescence method with the non-immunofluorescence methods,the formerhas a higher specificity and sensitivity for determining the Hp.
出处
《第四军医大学学报》
1995年第3期211-213,共3页
Journal of the Fourth Military Medical University
关键词
免疫荧光法
幽门螺杆菌
尿素酶
消化性溃疡
胃炎
immunofluorescence
Helicobacter pylori
urease
peptic ulcer
chronic gastritis
