摘要
用逆转录－聚合酶链反应（ＲＴ－ＰＣＲ）检测了４２例肝细胞癌（ＨＣＣ）患者肝癌及癌旁肝组织中丙型肝炎病毒（ＨＣＶ）ＲＮＡ的正、负链。结果显示：肝组织中ＨＣＶＲＮＡ正、负链的检出率分别为５４．８％和３５．７％；ＨＣＶＲＮＡ的正链和负链在肝癌和癌旁肝组织中，同时阳性的患者分别占４５．２％和３１．０％；血清ＨＣＶＲＮＡ和抗ＨＣＶ阴性者肝组织中ＨＣＶＲＮＡ的检出率仍有５１．３％；肝组织ＨＣＶＲＮＡ与乙型肝炎病毒（ＨＢＶ）的感染状态无显著相关。提示，ＨＣＶ感染可能是ＨＣＣ发生的另一病原学因素；血清ＨＣＶＲＮＡ和抗ＨＣＶ阴性时不能排除体内存在ＨＣＶ感染的可能性。
Liver samples from 42 patients with hepatocellular carcinoma(HCC) were examined for the presence of plus-strand and minus-strand HCV RNA by reverse transcription-polymerase chain reaction(RT-PCR).The results showed that the positive rates of plus-strand and minus-strand HCV RNA were 54.8% and 35.7% respectively.45.2 percent of the patients were found positive for the plus strand and 31.0 percent positive for the minus strand both in cancerous and non-cancerous liver tissues.Among those without detectable HCV RNA and anti-HCV,HCV RNA was detectable in liver tissues of 51.3% of the patients.No correlation was found between HCV RNA and HBV markers in the liver tissues and the sera.The findings suggest that HCV infection in China may play another etiological role in the development of HCC,and the presence of HCV infection cannot be excluded for lack of anti-HCV and HCV RNA in sera.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1995年第5期401-404,共4页
Academic Journal of Second Military Medical University
关键词
肝肿瘤
丙型肝炎病毒
逆转录
聚合酶链反应
liver neoplasms
hepatitis C virus
reverse transcription-polymerase chain reaction
