摘要
以１３１Ⅰ标记抗人肝癌单克隆抗体（Ｈｅｐａｍａ－Ⅰ，以下简称［１３１Ⅰ］－Ｈｅｐ）及正常小鼠ＩｇＧ（以下简称［１３１Ⅰ］－ｍＩｇＧ）进行裸鼠人原发性肝癌移植瘤的肿瘤定位显像研究。每鼠从尾静脉注射１．１１×１０７Ｂｑ，注入后２４，４８，９６，１６８ｈ行全身γ照相，观察肿瘤摄取标记抗体的能力，并计算生物半衰期，同时定期测定肿瘤与各器官组织的放射性比值（Ｔ／ＮＴ）。结果：［１３１Ⅰ］－Ｈｅｐ能选择性地积聚于肿瘤组织，其摄取率高于［［１３１Ⅰ］－ｍＩｇＧ（Ｐ＜０．０５），Ｔ／ＮＴ值在注入后９６ｈ最高，［１３１Ⅰ］－Ｈｅｐ的体内存留时间明显长于［１３１Ⅰ］－ｍＩｇＧ。提示Ｈｅｐａｍａ－Ⅰ在裸鼠体内对人肝癌细胞具有较高的亲和力，是一种有用的导向载体。
Tumor xenografts of human primary hepatic cancer (PHC) were grown in nude mice subeutaneously. The studies were performed in nude mice with tumor of base near 0.8~ 1.0 cm3. For the study of RII,the mice were injected intravenously with 1. 11×107Bq/mouse of [131I]-Hep. All tumors were clearly visualied at the 4th day after injection and remained the same at the 7th day. No tumor imasing was found in [131I]-mIgG.The radioactive ratio of tumor to liver increased from 1.3 to 3.03 in the [131I]-Hep group, but decreased from 13. 6 to 6.88 in the [131I]-mlgG group.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1995年第2期127-129,共3页
Academic Journal of Second Military Medical University
关键词
单克隆抗体
肝肿瘤
放射性核素
成像
antibody, monoclonal
liver neoplasms
radionuclide image
nude mice
