摘要
将ＨＦＲＳ病毒陈株可溶性抗原吸附于硝酸纤维素膜上，建立了用碱性磷酸酶免疫斑点试验（ＡＰ－ＤＩＢＡ）检测小鼠血清中的ＨＦＲＳ病毒抗体的方法，并与应用辣根过氧化物酶的免疫斑点试验（ＨＲＰ－ＤＩＢＡ）作了比较。结果表明，ＡＰ－ＤＩＢＡ具有特异性；其敏感性高于ＨＲＰ－ＤＩＢＡ。该法可用于不同ＨＦＲＳ病毒株感染小鼠血清中抗ＨＦＲＳ病毒抗体的检测。
A dot immunobinding assay with alkaline phasphatase(AP-DIBA)for detection of antibodies to HFRS virus was established by using the soluble antigens from HFRS virus(Chen Strain)whichwas absorbed to nitrocellu]ose filter as a solid carrier and was compared with the HRP-DIBA.It wasshowed that AP-DIBA was of specificity and higher sensitivity than HRP-DIBA.The AP-DIBA couldbe used for the determination of antibodies to HFRS virus in the sera from mice that were infected withdifferent strains of HFRS virus.
关键词
肾综合征出血热
出血热病毒
抗体
免疫斑点试验
hemorrhagic fever with renal syndrome
dot immunobinding assay
antibody detection
