摘要
本文介绍一种柯萨奇B_5病毒感染的搏动乳鼠心肌细胞微量模型。以400TCID_(50)/0.05ml病毒感染心肌细胞,48h后出现明显细胞病变,心肌释放酶(LDH及SGOT)明显升高,DNA合成速率明显下降;96h后,受染细胞培养液中产生Lg6.95TCID_(50)的病毒,细胞质出现特异性荧光着色。该模型证实,柯萨奇B_5病毒可在鼠心肌细胞中复制、装配并释放;同时也为诊治病毒性心肌炎的各种研究提供了工具。
A micro-model of Coxsackie B5 virus infected rat beating heart cells was introduced.In the infected group, the cardiac enzymes-lactic acid dehydrogenase (LDH) and glutamic oxaloacetic transaminase(SGOT)werc significantly increased and the synthesis rate of DNA was decreased 48 h after inoculation with 400 TCID50 Coxsakie B6 virus.Virus titer(Lg 6.95 TCID50)in the supernatant of the infected cultures was detected 96 h after virus challenge.In addition, the specific fluorescence in cytoplasma of infected cells could be observed.These results suggest that Coxsackie B5 virus might be replicated, assembled and released in cultured rat myocardial cells.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1989年第3期248-250,256,共4页
Academic Journal of Second Military Medical University
关键词
柯萨奇病毒
心肌细胞
细胞培养
Coxsackie B3 virus
myocardial cells
cell culture
