摘要
快速ELISA的建立和应用皮国华,邓瑶,刘加,王慧娟,张素香(中国预防医学科学院病毒学研究所,北京100052)关键词快速ELISA,抗-重组甲肝病毒单克隆抗体,抗-HBs单克隆抗体1972年Engvall等人发展了酶联免疫吸附试验(Enzyme-l...
n efficient rapid shaking incubation system was improved for the detection of HBsAgand hybridoma cell lines producng monoclonal antibodies against recombinant hepatitis Avirus.Each step of the reaction can be completed in 30 minutes at 37℃.This shakingincubation system is a sensitive and rapid test which has been applied to produce kits for diagnosis of clinical diseases.The optimized shaking system permits economical use of reagentssince the coating antibody can be used at higher dilution than in static incubator.
出处
《病毒学报》
CAS
CSCD
北大核心
1995年第4期381-383,共3页
Chinese Journal of Virology
关键词
ELISA
免疫学检验
应用
Hepatitis B virus surface monoclonal antibody,Recombinant hepatitis A virusmonoclonal antibody,Rapid enzyme-linked immunosorbent assay