摘要
应用Ｎｏｒｔｈｅｒｎ杂交技术发现遗传听源惊厥易感大鼠Ｐ７７ＰＭＣ在出生１４天后脑内ｃ－ｆｏｓｍＲ－ＮＡ水平迅速增高，２１天达到最高水平，而后逐渐下降，此与大鼠惊厥易感性形成规律相类似；惊厥后脑内ｃ－ｆｏｓｍＲＮＡ具有快速、大量及一过性表达特点；惊厥前后１５分钟内给予安定（１０ｍｇ／ｋｇ）能有效阻断ｃ－ｆｏｓ基因表达。推测ｃ－ｆｏｓ基因间接地参与了Ｐ７７ＰＭＣ大鼠惊厥易感性的形成。
e found that the basal c-fos mRNA level in the brain of P77 PMC rat(the audiogenic seizure-prone rat model)was rapidly reached the highest level on the 21st day after birth,then decreased to the same level with adult rat,which was correlated with the most susceptible period of audiogenic seizures.The character of c-fos mR NA expression in the brain of P77 PMC rat was rapid,large and transient after audiogenic seizure, Diazepam(10mg/kg,ip)injected 15min before or after seizure can completely block the c-fos gene expression.We suggest that c-fos gene is involved in the development and maturation of the central nervous system.There may be some close relationship between c-fos gene and the seizure susceptibility of P77PMC rats.
出处
《北京医科大学学报》
CSCD
1995年第4期281-284,共4页
Journal of Peking University(Health Sciences)
基金
国家自然科学基金
关键词
遗传性
惊厥
C-FOS
基因表达
大鼠
Headings Convulsions/genet
Proto-oncogene proteins c-fos
Gene expression
