摘要
应用逆转录－多聚酶链反应（ＲＴ－ＰＣＲ）法检测实验感染ＨＥＶ猕猴急性期血清及粪便标本中ＨＥＶＲＮＡ，并与其血清ＡＬＴ异常和抗－ＨＥＶＩｇＧ阳转相比较，以了解猕猴感染ＨＥＶ后上述各项标志的动态变化规律。结果表明，ＨＥＶＲＮＡ血症多出现于ＡＬＴ升高之前，持续约１～２周，当血清抗－ＨＥＶｌｇＧ阳转时ＨＥＶＲＮＡ即转为阴性；粪便ＨＥＶＲＮＡ可与病毒血症同时出现，但持续时间较病毒血症为长，于感染后３～４周仍可检测到。
sensitive and specific RT-PCR
assay was used for the detection of HEV RNA in sera and faeces of
experi-mentally infected rhesus monkeys.The patterns of viraemia and
virus shedding in faeces were compared with the dynamics of ALT
elevation and seroconversion of anti-HFV IgG.The results showed that
HEV RNA in serum was detected before the elevation of ALT and
disappeared at or soon after seroconversion of anti-HEV IgG,with a
peri-od of 1-2 weeks.Excretion of HEV RNA in stools was coincident
with viraemia and remained positive for 2-4 weeks,indicating that HEV
shedding in faeces is more prolonged than viraemia.
出处
《北京医科大学学报》
CSCD
1995年第4期243-245,共3页
Journal of Peking University(Health Sciences)
基金
美国CMB资助
