摘要
植物原生质体在培养中的粘连程度与其质膜所带负电荷的多寡密切关联，较易发生原生质体粘连的作物如胡萝卜、谷子，其原生质体表面负电性一般较弱；相反，不易发生原生质粘连的作物如烟草等，其原生质体表面负电性一般较强。在培养基中外源添加一些可以增强或削弱植物原生质体负电性的物质，能够对原生质体的粘连情况产生明显的影响。
Aggregation of Protoplasts from Dan ons carota and Setaria italica Was observed in Protoplasts culture. This phenomenon did not happen in Nicotiana tabachm. The reason was investigated by running cell electrophoresis. The reslllts indicated that surface charge of protoplasts and aggregation were closely related. Protoplasts of tobacco with high negativecharge, because of electrostatic repulsion force,showed no tendency to aggregate. While protoplasts of carrot with low negative charge. because of electrostatic repulsion force not enough to overcome Van der Waals force,tended to aggregate. Thechange of surface charge by treating protoplasts with some additives in culture medium had an important effects on aggregation of plant protoplasts.
出处
《安徽农业科学》
CAS
1995年第3期197-198,196,共3页
Journal of Anhui Agricultural Sciences
关键词
植物
原生质体
粘连现象
质膜电性
电泳率
谷子
Protoplast culture,Cell aggregation,Surface charge, Electrophoretic mobility
