摘要
本文报道采用免疫组化ＡＢＣ法检测了８０例鼻咽活检组织的ＰＣＮＡ和Ｐ５３蛋白表达情况，结果显示：（１）ＰＣＮＡ和Ｐ５３表达的阳性率在鼻咽癌分别为９４２％和８４．６％，异型性改变为７０．０％和２８．８％，单纯性增生为３．９％和阴性，正常上皮均为阴性；（２）ＰＣＮＡ和Ｐ５３表达的细胞阳性强度和阳性细胞数量与鼻咽癌的组织类型无关，但ＰＣＮＡ和Ｐ５３表达的细胞阳性强度及Ｐ５３表达的阳性细胞数量与肿瘤的间变程度有关；（３）ＰＣＮＡ的中一强度阳性表达见于无论是癌旁或慢性炎来源的重、中度异型性改变上皮，但Ｐ５３的中一强度阳性表达仅见于癌旁来源的重、中度异型性改变上皮。结果提示：（１）ＰＣＮＡ和Ｐ５３表达的细胞阳性强度考虑可作为了解鼻咽癌恶性程度及患者预后的辅助指标；（２）在鼻咽癌前病变的研究方面，Ｐ５３蛋白的检测可能比ＰＣＮＡ的检测更有价值。
The expression of PCNA and p53 protein
were examined in 80 biopsies of nasopharyngeal lesiosby means of
immunoliistochemical ABC method。The results showed that: l) The PCNA
and p53 positive rates were 94.2%and 84.6 % in nasopnaryngeal
carcinoina (NPC),70.0%and 28.8%in dysplasia,3.9 % andnegative in
hypernlasia, and both negative in normal epithelia respectively,2) In
NPC,the cellular staining intensity of PCNA or p53 e xpression was
related to the exent of tumor anaplasia, but not to the histological
typing and the positive percentage of p53 expression also was related
to the extent of tumor anapiasia; 3)The moderate-intense cellular
statining of PCNA expression was in the moderate and severe dysplasi
a whetherfrom paracarcinoma一or chronic iiasopharyngitis
-epithelia,but the inoderate-intense cellular staining of p 53
expression was only in the moderate and seveere dysplasia from
paracarcinoma-epithelia The results suggest that:1)The cellular
staining intensity of PCNA and p53 exp-。 ss ion may be served as the
auxiliary indicators for identifying-the extent of NPC ma-lignancy
and predicting the prognosis of NPC patients,2)In study on precancer
lesion of NPC,it might be more valuble to testing p53 than PCNA
expression。
出处
《癌症》
SCIE
CAS
CSCD
北大核心
1995年第2期99-102,共4页
Chinese Journal of Cancer
关键词
鼻咽肿瘤
增殖细胞核抗原
P53蛋白
Nasopharyngeal carcinoma Nasopharngeal dyplasia
Prolifearingcell nuclear antigen p53 protein
