摘要
采用未照射（对照）和体外放射线照射３个供血员的全血，检测应用细胞松弛素法（ＣＢ）与常规法，对测得的淋巴细胞微核直径和微核率作了比较。结果表明：１）３．１Ｇｙ的γ－线照射，ＣＢ法微核率７．５１７％高于常规法６．１７１％（Ｐ＜０．０５）；ＣＢ法平均微核体积是常规法的１．８倍，ＤＮＡ损伤检测效率是常规法的２．２倍。２）未照射组中，ＣＢ法测的健康人自发微核率０．７６７％也高于常规法０．４９２％（Ｐ＜０．０５）；ＣＢ法平均微核体积是常规法的１．６倍，ＤＮＡ损伤检测效率是常规法的２．５倍。结合文献资料可认为，ＣＢ法在检测电离辐射的诱变性上较常规法敏感。细胞松弛素Ｂ有一定的遗传毒性，实际工作中应有选择地使用ＣＢ法。
The frequencies and diameters of micronucleus in the cultured lymphocyte from the different proparations with cytokinesis-block(CB-MNT)and conventional method(C-MNT)were measured and compared.Results showed that:1)the frequencies of the mi-cronucleus induced by 3.1Gy gamma ray irradiation were:7.517%in CB-MNT and 6.171%in C-MNT,P<0.05.The average diameter of the micronucleus was 1.8 times larger in CB-MNT than C-MNT。The efficiency in detecting damaged DNA was 2.2 times greater in CB-MNT than C-MNT,2)Same trend ws found in the negative control groups for sponta-neously occured micronucleus,0.767%vs 0.492%in frequency,1.6folds in diameter,and 2.5times in efficiency detecting the damaged DNA. Results in dicated that the CB-MNT is more sensitive than C-MNT。However as Cytochalasin-Bitself is a clastogen,CB-MNT should be used selectively and with its results explained cautiously。
出处
《癌变·畸变·突变》
CAS
CSCD
1995年第6期373-376,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
关键词
细胞分裂阻滞
微核测试法
微核率
诱变监测
Cytochalasin-B
Micronucleus
Frequency of micronucleus
Diameter of mi-cronuleus
Efficiency detecting damaged DNA
