摘要
本文用姐妹染色单体互换及非同位素原位缺口平移技术观察了Ｎ－甲基－Ｎ’－硝基－Ｎ－亚硝基胍（ＭＮＮＧ）对人支气管上皮永生细胞系的遗传毒理作用。结果表明ＭＮＮＧ可致人支气管上皮永生细胞系ＤＮＡ损伤，并以ＤＮＡ单链断裂的形式出现。上述方法的建立为人类致癌物的检测及其作用机理的研究建立了有效的实验模型。
In the immortalized human branchial epithelial cells,the gentoxic effects induced by N-Methyl-N-Nitro-N-nitrosoguanidine(MNNG)were observed with the methods of sister chromatid exchnage and nonradioactive in situ nick translation。The results showedthat MNNG can induce DNA damage of the cell by the way of DNA single strand break.This model system is very useful in detecting carcinogen and carcinogenesis in human being。
出处
《癌变·畸变·突变》
CAS
CSCD
1995年第6期329-331,共3页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
医科院CMB基金
关键词
MNNG
遗传毒理
永生细胞系
支气管
immortalized cell line
sister chromatid excliange
in situ nick translation
DNAdamage
