摘要
利用ＨＰＬＣ（高效液相色谱）电化学检测法分析ＤＮＡ内８—羟基—２’—脱氧鸟苷（Ｏｈ８ｄＧ）的含量是一个定量分析ＤＮＡ氧化性损伤的有效方法。研究发现大鼠经口给予溴酸钾（ＫＢｒＯ３）能在其靶器官肾脏产生ｏｈ８ｄＧ；硫酸锂能有效保护大鼠肾ＤＮＡ免遭ＫＢｒＯ３的氧化损伤，未见保护作用有剂量—反应关系。
Measurement of 8 hydroxy 2’ deoxyguanosine(oh8dG)in DNA by HPLCwith electrochemical detection Provides an useful method for the quantitative analysis of ox-idative DNA damage. It was found that oral administration of potassium bromate(KBrOa)specifically produced oh8dG in rat target organ DNA. Whereas Li2SO4 could substantially pro-tect DNA of rat kidney from oxidative damage by KBrO3. However,dose response relation-ship was not found in this experiment。
出处
《癌变·畸变·突变》
CAS
CSCD
1995年第1期12-15,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
关键词
DNA氧化损伤
保护作用
卫生毒理
oh^8dG
hydroxy 2’ deoxyguanosine
Oxidative DNA damage
High pressure liquidchromatography
Protection effect
