摘要
作者以人雌激素受体Ｄ段（ＥＲ－Ｄ）含１５个氨基酸的合成肽与匙孔血蓝蛋白（ＫＬＨ）的交联物为抗原免疫雄性小鼠，制备抗ＥＲ－Ｄ抗血清，并以此用免疫组织化学（ＩＨＣ）技术对９５例经葡聚糖包裹活性碳（ＤＣＣ）法分析的乳腺癌组织切片进行ＥＲ检测。同时，首次采用地高辛配基标记探针的非同位素原位杂交（ＩＳＨ）技术，检测了１５例同一批标本的ＥＲｍＲＮＡ表达水平。实验结果显示：ＩＨＣ和ＤＣＣ之间，其阳、阴性符合率为９０％，相对于ＩＨＣ的半定量分级而言，其程度符合率为６９％，ＩＨＣ与ＩＳＨ的阳、阴性符合率达１００％。因此，作者认为，抗ＥＲ－Ｄ抗血清的ＩＨＣ法可以代替或辅助ＤＣＣ法用于临床乳腺癌ＥＲ的检测。
Abstract An antiserum to peptide containing 15 amino acids
corresponding to the region-D of human e-strogen receptors(hERD)was
obtained in mice by immunization with the peptide conjngated
toKLH.Using this antiserum,the ER status of paraffin-embeded sections
of 95 human breast car-cinomas(in which,sections of 31 were both
frozen and paraffin-embeded ones)were studied.The corresponding rate
for determination of ER status between immunohistochemical
staining(IHC)and dextran coated charcoal(DCC)assay was 89.5%.The
concordance rate for semiquan-titative gradings was 69.3%.In
addition,in situ hybridization(ISH)of 15 frozen sections of
thesamples using digoxigenin labeled dUTP to identify the expression
of ER mRNA was also done.The result of ISH was fully consistent with
that of IHC(100%).The results show that themouse antiserum to hERD
obtained in this study is specific and sensitive for IHC assay of ER
andIHC is a valuable adjunct and/or alternative to the biochemical
method for determination of theER status of breast cancer.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
1995年第5期350-353,I007,T001,共6页
Chinese Journal of Oncology
