摘要
采用Ｔｉ质粒的双元载体法对西红柿（Ｌｙｃｏｐｅｒｓｉｃｏｎｅｓｃｕｌｅｎｔｕｍ）的叶片外植体进行遗传转化，经选择培养基的筛选，从抗性愈伤组织细胞中诱导出具有卡那霉素抗性的幼苗．分子杂交证实，卡那霉素抗性基因通过根瘤农杆菌（Ａｇｒｏｂａｃｔｅｒｉｕｍｔｕｍｅｆａｃｉｅｎｓ）的介导，整合到细胞核基因组中，转化子具有较高的新霉素磷酸转移酶活性，说明抗性基因在受体细胞得到表达．
Kanamycin resistant calli are obtained by transferring tomato (Lycopersicon esculetum) leaf explants and using Ti plamid binary vector system. Resistant shoots are induced from the calli. Southern blots confirm the incorporation of kanamycin resistant gene into the plant genome. High neomycin phosphotransferase activity in the transformants shows expression of the resistant gene in the recipient cells.
出处
《陕西师大学报（自然科学版）》
CSCD
1995年第3期71-74,共4页
Journal of Shaanxi Normal University(Natural Science Edition)
基金
国家自然科学基金
关键词
双元载体
抗性基因
转化
细胞
蕃茄
tomato
binary vector
resistant gene
transformation
