摘要
用转铁蛋白受体（ＴＦＲ）单抗ＯＫＴ９，以免疫电镜技术，对肝细胞癌（ＨＣＣ）ＴＦＲ的表达作亚细胞定位研究。结果表明，ＴＦＲ电镜下呈电子密度较高的颗粒状物质。肝癌细胞大量表达，正常肝细胞不表达或少量表达。肝癌细胞的ＴＦＲ分布在胞膜，但更多地分布在胞浆。从而证实先前定性与定量的研究结果。并根据ＴＦＲ所在位置，就其在细胞内的动态过程作了探讨。
sing monoclonal anti-transferrin
receptor(TFR)antibody OKT9,TFR in hepatocellular carcinomawas
ultrastructurally localized by means of immunoelectron microscopical
technique. Results indicatedthat under electron microscope, TFR
appeared as granular material with higher electronic density. Agreat
number of TFR were found in liver cancer cells, but a few or even no
in normal liver cells. Immunoreactivity to TRF was localized
dominantly in cytoplasm on rough endoplasmic reticulum,
smallvesicles, and coated pits,occasionally was found on microvilli
in canalicular lumina,The result demon- strated our previous
qualitative and quantitative studies.The kinetics of TFR in liver
cancer cell was also discussed on the basis the localization of TFR.
基金
国家自然科学基金
关键词
肝细胞癌
转铁蛋白
受体
免疫电镜
Hepatocellular carcinoma Transferrin receptor Immunoelectron
microscopic technique Ultrastructural localization of receptor
