摘要
以胶体金标记抗病毒交联物Ｌ－ＨＳＡ－Ａｒａ－ＡＭＰ，做直接胶体金银染色，光镜观察该交联物识别的去唾液酸糖蛋白受体在大鼠肝细胞及ＨｅｐＧ－２细胞表面的定位，同时分别用大鼠脾组织，金标ＨＳＡ做对照。结果：去唾液酸糖蛋白受体多分布在大鼠肝细胞的血窦面，侧面亦有少量分布。ＨｅｐＧ－２细胞表面受体呈阳性。而两组对照组受体标记均呈阴性。
o observe the distribution of asialoglycoprotein recepto(ASGP-R)on the cell surface of rat liver cells and hunan hepatoma cells(HepG-2 cell),the antiviral conjugate, lactosaminated human serum arabinoside adenine monophosphate(L-HSA-Ara-AMP),were labelled with colloidal gold,and then the direct gold-silver staining was employed.The rat spleen and colloidal gold labelled HSA were used as controlled groups.The results indicated :ASGp-R were present on the surface of rat liver cells and HepG-2 cells.In the former,most AsGp-R are along the sinusoidal margin and partly on the lateral surface of hepatocyte.While the two controlled groups were negative.
出处
《肝脏病杂志》
CSCD
1995年第3期170-172,共3页
