摘要
应用免疫组织化学方法对一组肝病患者石蜡包埋肝组织中丙肝病毒抗原进行了检测。２５２份肝组织中，采用多克隆抗ＨＣＶ检出ＨＣＡｇ阳性２５份（９．９２％），采用单克隆抗ＨＣＶ－ＮＳ３检出ＨＣＶ－ＮＳ３蛋白阳性１９份（７．５４％）。１９份ＨＣＶ－ＮＳ３阳性肝组织均同时表现ＨＣＡｇ阳性，二者在肝细胞分布状况相似。另有６例肝组织中检出ＨＣＡｇ阳性，阳性细胞周围多无坏死灶和炎性。不同病理类型肝炎患者肝组织中丙肝病毒抗原检出率无明显差异。肝组织中抗原的检出和血清ＨＣＶ标志具有良好对应性，但不同血清ＨＣＶ标志模式抗原检出率存在明显差异。２５２份对应血清检出ＨＣＶ标志７１例，单独抗ＨＣＶ阳性者无一例肝组织中检出抗原，血清抗ＨＣＶ和ＨＣＶＲＮＡ均阳性者较单一，ＨＣＶＲＮＡ阳性者的抗原检出率低。有二例肝组织中检出抗原但其血清ＨＣＶ标志阴性。
epatitis C
virus antigens in paraffin-embeded liver tissues of patients with
liver disease were detected byimmunohistochemistrv method to study
its expression and clinical significance. HCAg and HCV-NS3 protein
weredetected by using polyclonal anti-HCV and monoclonal anti-HCV-NS,
and the positive rate were9.92%(25/252)and
7.54%(19/252),respectively.All of the nineteen HCV-NS3 positive
tissues were also positive for HCAg.The distribution of them in liver
cells was similar.Another six samples were HCAg positive but
HCV-NS3negative. There were no obvious necrosis and inflammation
aggregaeion around the positive cells in most of
thesections.Significant difference of the positive rate of HCV
antigen in liver tissues was found among differentmodels of serum HCV
markers but not among different pathologic type of hepatitis.No HCV
antigens weredetected in the liver of patient with anti-HCV positive
only.Still,the positive rate was much lower in the liverof patient
with both serum anti-HCV and HCV RNA positive then that of patient
with serum HCV RNA positiveonly. We considered that detection of HCV
antigen in liver tissue using immunohistochemistry method supplysome
help for understanding the pathogenesis of HCV.
出处
《肝脏病杂志》
CSCD
1995年第3期158-160,共3页
关键词
HCV
抗原
肝炎
肝病
HCV HCV
antigen HCV RNA Immunomstochemisty
