摘要
采用ＨＣＶＣ区不同表位抗原研制的试剂盒，检测１４例首次ＨＣＶ感染者不同时期的１２９份系列血清中抗体。重组表达的ＨＣＶＣ区抗原及合成肽均与系列血清抗体有较好的反应性。ＨＣＶＣ区不同表位的抗体反应可以同时出现或先后出现，其动态变化规律并不一致。含ＨＣＶＣ区亲水和疏水部分重组抗原研制的试剂，其早期抗体检出率显著地高于仅选部分亲水区抗原包被试剂的检出率，提示重组高效表达的ＨＣＶＣ区抗原在输血后Ｃ型肝炎患者的早期诊断和献血员的筛选中有重要意义。
29 samples of serial sera from l4 patients
with post-transfusion hepatitis C(PTHC)were detected for anti- HCV by
ELISA using of series of HCV antigens with different epitopes。Marked
reactivities have been found on those of recombinant or synthetic
antigens。Antibodies to different epitopes of core protein of HCV
could appear simultaneously or sequentially。 With test kits of
anti-HCV consisted of C11 antigen bearing both hydrophilic and
hydrophobic regions, the detective rate was remarkdly higher than
those with that only bearing hydrophilic regions in early detection
of PTHC. The result sugested that re- combinant core protein is
recommendable in early dignosis of PTHC.
