摘要
应用夹心ＥＬＩＳＡ检测了５６例乙型病毒性肝炎患者外周血单个核细胞（ＰＢＭＣｓ）白细胞介素－６（ＩＬ－６）的诱生水平。乙型慢性活动性肝炎（ＣＡＨ）、乙型肝炎后肝硬化（ＨＣ）和乙型重型肝炎（ＳＨ）ＰＢＭＣｓ经脂多糖诱导后，ＩＬ－６的诱生水平分别为１２．８４±３．２６ｎｇ／ｍｌ、１１．７９±３．０１ｎｇ／ｍｌ和３８．４２±８．３７ｎｇ／ｍｌ，明显高于正常对照组（Ｐ＜０．０５～０．０１）。ＣＡＨ和ＳＨ患者的ＩＬ－６水平与肝细胞变性坏死，蛋白质合成障碍和患者的预后也有一定的关系。上述结果提示，机体内ＩＬ－６的调节紊乱可能与病毒性肝炎的发病机理有关。
Interleukin-6 (IL-6) induced from peripheral mononuclear cells (PBMCs) of 56 patients with viral hepatitis B was detected by sandwich ELISA. IL-6 levels in patients with chronic active hepatitis (CAH), hepatic cirrhosis (HC) and severe hepatitis(SH) were 12.84±3.26ng/ml,11. 79±3. 01ng/ml and 38.42±8. 37ng/ml, respectively,which were significantly higher than that of the normal controls. Moreover,the changes of IL-6 level in CAH and SH were positively correlated with the liver function abnormalities and prognosis. Based on these findings,it may be stated that the elevation of IL-6 level is related to the pathogenesis of viral hepatitis B.
出处
《标记免疫分析与临床》
CAS
1995年第1期12-14,共3页
Labeled Immunoassays and Clinical Medicine
关键词
乙型肝炎
白细胞介素6
ELISA
Hepatitis B
Liver cirrhosis
Interleukin-6
Enzyme-linked immunosorbent assay
