摘要
实验研究了使用阳离子表面活性剂Aliquat336与异辛烷构成的反胶团溶液，通过液－液萃取方式纯化工业级α-淀粉酶的过程．当使用的反胶团溶液组成为Aliquat336／isooctane／1％（V／V）n－butanol时，实验发现纯化工业级α-淀粉酶的最佳条件：水相组成为30mmol／L，pH10，或酶，萃取时间为1min；反萃液组成为30mmol／L，pH6，反萃时间为3min在此条件下，通过萃取循环能够达到纯化工业级α-淀粉酶的目的，经过一个萃取与反萃循环后，α-淀粉酶的比活提高1．5倍，酶活回收率达85％与此同时，工业级α-淀粉酶中所含的中性蛋白酶活回收率只有10％，而且其比活降低了近7倍。α-淀粉酶与中性蛋白酶间的分离系数能达10左右．
Purification of industrial α-amylase was carried out by using liquid-liquid extraction with Aliquat 336/isooctane/1% (V/V) n-butanol reversed micellar solution as the extractant.The optimal experimental conditions to purify industrial α-amylase were found:the compositions of initial aqueous phase is 30 mmol/L buffered solution at pH=10 colltaining crude enzyme, the forward extraction time 1 min; the composition of stripping solution is 30 mmol/L of reversed micelles in buffered solution at pH=6, the backward extraction time 3 min.Under such conditions, 85% of the total activity of α-amylase in the crude enzyme preparation can be recovered after a full forward and backward extraction cycle and α-amylase purified about 1.5 fold.Meanwhile most of the neural protease in the industrial grade o-amylase could be removed at the end of an extraction cycle. The separation factor of α-amylase to neutral protease is upto about 10.
出处
《化工冶金》
CSCD
北大核心
1995年第3期229-234,共6页
基金
国家自然科学基金
关键词
反胶团
纯化
Α-淀粉酶
萃取
发酵
Aliquat 336, Reversed micelles, Purification, α-amylase
