摘要
超氧化物歧化酶明胶微球的制备及性质邹国林，徐传斌，胡萍，李鹏（武汉大学生命科学学院，武汉，４３００７２）关键词超氧化物歧化酶，明胶微球，稳定性，免疫原性中图法分类号Ｑ５５１，Ｒ９４３超氧化物歧化酶（ＳＯＤ）是一种以超氧阴离子自由基为底物的酶，亦是一种...
Coppet/zinc superoxide dismutase from bovine erythrocytes has been isolatedand purified. The specific activity of the purified enzyme is 8891 units per mg protein.lt exhibits one absorption maximum in the ultraviolet at 265 urn. A practical and simple methodfor the preparation of gelatin microspheres containing SOD is reported. The average diameterof the microspheres is 24 pm. There is 79. 6% recovery of SOD activity from the preparationof the microspheres. The experimental results prove that SOD entrapped in the microspheresexhibits more stabilities towards temperature,pH and hydrolysis of trypsin and similar immunogenicity as compared with natural SOD. As a consequence,the SOD microspheres canbeneficially be used as an enzyme for pharmaceutical .food and cosmetic industry.
出处
《武汉大学学报（自然科学版）》
CSCD
1995年第4期517-520,共4页
Journal of Wuhan University(Natural Science Edition)
关键词
超氧化物歧化酶
明胶微球
稳定性
微球
制备
superoxide dismutase,gelatin microsphere, stability,immunogenicity
