摘要
研究了创伤小鼠活化Ｔ细胞内游离钙浓度（［Ｃａ２＋］ｉ）、钙调素（ＣａＭ）、钙调素依赖的蛋白激酶（ＣａＭ－ＰＫ）活性的变化及其与Ｔ细胞功能之间的关系。结果显示，创伤后活化Ｔ细胞内［Ｃａ２＋］ｉ降低，且这一变化同创伤小鼠Ｔ细胞白介素２（ＩＬ－２Ｒα）ｍＲＮＡ、ＩＬ－２受体α（ＩＬ－２Ｒα）ｍＲＮＡ水平降低，ＩＬ－２生成减少，ＩＬ－２Ｒα表达受抑，Ｔ淋巴细胞转化（ＴＬＴ）降低密切相关。创伤后活化Ｔ细胞内ＣａＭ、ＣａＭ－ＰＫ活性明显降低。钙离子导入剂Ａ＿（２３１８７）在体外可提高创伤小鼠活化Ｔ细胞内［Ｃａ２＋］ｉ至正常对照水平，并明显提高其ＣａＭ、ＣａＭ－ＰＫ活性及ＩＬ－２ｍＲＮＡ、ＩＬ－２ＲαｍＲＮＡ水平，但不能使之恢复正常。提示活化Ｔ细胞内［Ｃａ２＋］ｉ、ＣａＭ、ＣａＭ－ＰＫ活性降低是导致创伤后Ｔ细胞功能受抑的重要原因。
The changes of free calcium level(Ca2+).calmodulin(CaM)and CaM-dependent protein kinase(CaM-PK)activity in the activated T-cells after trauma and their relationship with T-cell functions were studied in mice.It was found that Ca2+in activated T-cells was reduced after trauma,which was closely related to the reduction of interleukin 2(IL-2)mRNA and IL-2 receptor alpha mRNA levels,a decrease of IL-2 production,a suppression of IL-2 receptor alpha expression and a reduction of T-cell transformation in T-cells,CaM and CaM-PK activities in the activated T-cells were significantly decreased after trauma.Calcium cation ionophore A23187 could restore Ca2+level in activated T-cells of traumatized mice to the control level in vitro and it could also inncrease CaM and CaM-PK activities and IL-2 mRNA and IL-2 receptor alpha mRNA levels significantly though they were still under the normal levels.It is suggested that the reduced Ca2+and CaM and CaM-PK activities are the important factors to result in the suppression of T-cell functions after trauma.
出处
《第三军医大学学报》
CSCD
北大核心
1995年第3期214-216,共3页
Journal of Third Military Medical University
基金
国家自然科学基金
关键词
创伤
损伤
T-淋巴细胞
钙
钙调素
蛋白激酶类
wounds and injuries
T lymphocytes
calcium
calmodulin
protein kinases
