摘要
以ＡｎｇｒａｙＳＫ和自行设计的引物作半套式ＰＣＲ，成功地从人外周血Ｂ淋巴细胞中扩增出Ｉｇ重链可变区基因。扩增产物的分子量为６６０ｂｐ左右。该文结果提示，在建立全套抗体基因文库时，半套式ＰＣＲ法能较好解决Ｉｇ可变区基因多样性的扩增问题。
The variable region genes of the human immunoglobulin(Ig)heavy chains of peripheral Bcells were amplified with half-nested PCR using the primers designed by SK Angray and the authors themselves.The molecular weight of the PCR product was about 660 bp,The results suggest that when the library of the repertoires of antibody genes in to be constructed,half-nested PCR is useful to maximize the diversity of variable region genes of human Ig heavy chains.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1995年第3期206-208,共3页
Journal of Third Military Medical University
