摘要
在本研究中观察了一定剂量范围（２．５０×１０^（－４）～２．５×１０^（－７）ｍｏｌ／Ｌ）视黄酸（ｒｅｔｉｎｏｉｃａｃｉｄ．ＲＡ）对淋巴细胞膜上的转铁蛋白受体（ＣＤ７１）ｍＲＮＡ的影响。结果显示：大剂量ＲＡ（２．５×１０^（－４）ｍｏｌ／Ｌ）显著抑制ＣＤ７１ｍＲＮＡ产生，且这种抑制作用不能被加入的外源性人重组白介素２（ｒｈＩＬ－２）所逆转；２．５×１０^（－５）～２．５×１０^（－６）ｍｏｌ／ＬＲＡ则能增加ＣＤ７１ｍＲＮＡ含量，２．５×１０^（－７）ｍｏｌ／ＬＲＡ作用不明显（与二甲基亚砜（ＤＭＳＯ）溶剂对照比）。提示大剂量ＲＡ可能直接在基因转录水平上抑制了ＣＤ７１ＤＮＡ转录为ｍＲＮＡ。
The effects of large or physiological dosage of retinoic acid(RA) on the CD71 mRNA Ievel in the lymphocytes were observed with Dig labelled CD71 cDNA probe,It was found that 2.5×10-4mol/L RA could inhibit dramatically the production of CD71 mRNA,which was irreversible when external recombinant human interleukin-2 was added. Nevertheless,2.5×10-5 to 2.5×10-6 mol/L RA could increase the CD71 mRNA content in the lymphocytes and 2.5×10-7 mol/L RA induced a same response as that of the control with DMSO soluent. Our findings suggest that RA in large dosage can inhibit the transcription of CD71 DNA into mRNA.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1995年第2期134-136,共3页
Journal of Third Military Medical University
