摘要
研究苯酚在预活化聚酰胺膜酪氨酸酶生物传感器上的响应特性，适量儿茶酚的存在可加快传感器到达恒态的速度。被固定酶的量、活性及传感器的有效面积等是影响检测灵敏度的主要因素。首次采用预活化聚酰胺膜固定酪氨酸酶，并与浸蜡石墨电极组合成酪氨酸酶生物传感器，在温度２５℃电位－０．２００ＶｖｓＳＣＥ，儿茶酚浓度５×１０（－７）ｍｏｌ／Ｌ，底液０．１ｍｏｌ／Ｌ磷酸盐缓冲溶液ｐＨ６．５０下，采用电流法测定苯酚及水样中的酚。酶膜的制备、替换和贮存极为方便，酶膜贮存５个月未见活性下降，有良好的重现性和线性范围２×１０（－７）－１．２５×１０（－５）ｍｏｌ／Ｌ。
he response time decreases in the presence of catechol. The principal factors influencing the sensitivity were described. The amperometric biosensor of tyrosinase was prepared firstly by immobilizing tyrosinase onto preactivated polyamide support which contacted tightly with wax-impregnated graphite elbotnde.Amperotnetric measurements of phenol in water were carried out by applying a potential of-0. 200 VvsSCE in a 0. 1 mol/L phosphate buffer of PH6. 50 and 5×10-7 mol/L catechol at 25℃. The preparation, storage and replacement of immobilized enzyme are convenient. No activity of immobilized enzyme was lost during five months of storage, with linear range of 2×10-7-1. 25×10-5 mol/L of phenol.
出处
《环境科学》
CAS
CSSCI
CSCD
北大核心
1995年第3期1-3,共3页
Environmental Science
基金
江苏省教委自然科学基金
