摘要
应用鼠伤寒沙门氏菌／微粒体酶试验，选用ＴＡ９８及其两个新的对ＮＯ２－ＰＡＨｓ敏感的衍生菌株：富含硝基还原酶（ＮＲ）的ＹＧ１０２１和富含Ｏ－乙酰基转移酶（ＯＡＴ）的ＹＧ１０２４，对沈阳市不同功能分区１９９０年冬季大气悬浮颗粒物二氯甲烷提取物（以下简称提取物）进行直接致突变研究。结果表明商业区、工业区、居民区和风景区提取物对三个菌株均有直接致突变作用，呈剂量－反应关系。提示颗粒物中富含ＮＯ２－ＰＡＨｓ，而且上述的前三个区颗粒物中以二硝基芘（ＤＮＰｓ）为主要致突变物，同时也有１－硝基芘（１－ＮＰ）。风景区ＤＮＰｓ污染略低，１－ＮＰ与ＤＮＰｓ污染水平基本相同。ＤＮＰｓ污染以商业交通区最多，居民区及工业区次之，风景区较轻。
The mutagenicity of methylene chloride extracts of airborne particulates from defferential function destricts in Shenyang was studied with Salmonella typhimurium strain TA98 and its'classical' nitroreductase-overproducing derivative YG1021、and YG1024,another new derivative of TA98 with elevated O-acetyltransferase level.The extracts of particulates from commercial,industrial,residential and scenic ditricts showed direct and dose-dependent mutagenicity for the three strains.The revertants of the extracts of particulates from commercial,industrial and residential districts in YG1024 and YG1021 were higher than that in the parent strain TA98,and strain YG1024 was more sensitive than YG1021.The revertants of extracxts of particulates from scenic district in YG1024 and YG1021 were similar but higher than that in strain TA98.Samples from commercial district contained more dinitropyrenes(DNPs)than that from residential and industrial districts,and that from scenic district which contained the least DNPs among the airbore particulates from the four districts.This observation strongly suggests the presence of direct-acting mutagens NO2-PAHs,besides PAHs,in the airborne particulates from differential function districts in Shenyang and that DNPs must be the major direct-acting mutagens in these particulates.
出处
《环境与健康杂志》
CAS
CSSCI
CSCD
北大核心
1995年第3期104-106,共3页
Journal of Environment and Health
关键词
大气悬浮颗粒物
致突变性
AMES试验
硝基多环芳烃
Airborne particulate
Mutagenicity
Ames test
Nitrated polycyclic aromatic hydrocarbons (NO_2-PAH_s)
