摘要
从牙周病厌氧菌（Ｂａｃｔｅｒｏｉｄｅｓｇｉｎｇｉｖａｌｉｓ）单克隆抗体的杂交瘤细胞株出发，通过基因工程方法──ＰＣＲ技术，调出其抗体的重链和轻链基因可变区序列，体外重组后得到单链抗体ＳｃＦｖ（ｓｉｎｇｌｅｃｈａｉｎｆｒａｇｍｅｎｔｖａｒｉａｂｌｅ）片段，克隆到了ｐＣＡＮＴＡＢ５载体上，在ＥｓｃｈｅｒｉｃｈｉａｃｏｌｉＴＧｌ中得到了成功表达。
The hybridoma producting Anti-Bacteroldes gingivalis(Bg) antibody wasused as a begining strain. With PCR technology, the antibody heavy and light chain vari-able domains have been got, and was comprised them linked into a single chain bv a flexi-ble peptides which termed ScFv(single chain fragment of variable).The ScFv was clonedin vector pCANTAB5 and expressed in E, coli TG 1. The product of ScFv expressed ap-peared biological activity detected by ELISA method.
出处
《云南大学学报（自然科学版）》
CAS
CSCD
1995年第3期254-258,共5页
Journal of Yunnan University(Natural Sciences Edition)
关键词
牙周病厌氧菌
单链抗体
基因工程
口腔病
无性系
Bacteroldes gingivalis(Bg),PCR, ScFv(single chain fragment of varia-ble),clone,express
