摘要
为了探讨视网膜增殖性病变的发病机理，作用采用放射受体法检测了原代培养的人眼和猴眼视网膜色素上皮（ＲＰＥ）细胞无血清培养液的表皮生长因子（ＥＧＦ）活性。首次发现培养的人ＲＰＥ及猴ＲＰＥ细胞具有产生和分泌ＥＧＦ的活性。分泌高峰值人ＲＰＥ细胞为２．１１±０．４６ｎｇ／ｍｌ；猴ＲＰＥ细胞为１．５５±０．１２ｎｇ／ｍｌ。表明ＲＰＥ细胞是眼内ＥＧＦ的一个重要来源；ＲＰＥ可能通过自分泌和旁分泌机制在视网膜增殖疾病的发生发展中起重要作用。
The epidermal growth factor bioactivity from cultured primary human and monkey retinal pigment epithelial (RPE) cell systems was detected by using the radioreceptor assay. We report that the cultured human and monkey RPE cells produce and secrete the EGF bioactively to the media as demonstrated by radioreceptor binding assay. The EGF bioactivities secreted by human and monkey RPE cells were at peak of 48 hours (human RPE cells secreted 2. 11±0. 46ng/ml vs monkey RPE was 1. 56±0.12ng/ml) in the serum-free media.The results indicate that the RPE is one of important sources for EGF in the eye. The RPE cells may play much important roles in the development of proliferative retinal diseases through the autocrine or paracrine mechanism. This new discovery will be helpful to elucidate the pathogenesis of proliferative retinal diseases and also provide an important basis for the treatment of such diseases.
出处
《中华医学杂志》
CAS
CSCD
北大核心
1995年第10期609-610,共2页
National Medical Journal of China
关键词
视网膜色素上皮
表皮生长因子
视网膜疾病
Epidermal growth factor Retinal pigment epithelium Eyes Cell culture Radioreceptor assay
