摘要
应用逆转录／多聚酶链反应（ＲＴ／ＰＣＲ）及双扩增ＰＣＲ方法检测了４４例慢性期、缓解期及加速（或）急变期慢性粒细胞白血病患者的ｂｃｒ／ａｂｌｍＲＮＡ，所有患者均为阳性。各期患者均以表达ｂ＿３ａ＿２融合方式为主。１４例患者单用α－２ｂ干扰素或合用小剂量羟基脲治疗后，１３例患者ｂｃｒ／ａｂｌｍＲＮＡ仍阳性，有３例单次扩增结果阴性，提示白血病细胞负荷较低，１例孤立性骨髓外粒细胞白血肉瘤患者经治疗后复查ｂｃｒ／ａｂｌｍＲＮＡ转阴。但按作者应用的剂量及疗程对大多数患者尚难彻底清除残留病灶。
he bcr/abl fusion gene transcripts of 44 patients withchronic myelogenous leukemia were assayed by RT/PCRand nested PCR. The majority patients showed b_3a_2mRNA. The bcr/abl mRNA still can be detected in 13 out of14 patients treated with interferon alpha-2b or plus lowdose hydroxyurea, but the first round of amplificationshowed negative in 3 of these 13 patients,indicating thatthe leukemic cell loads were low in these patients. Onepatient with solitary extramedullary myelosarcoma with-out bone marrow involvement become bcr/abl mRNAnegative after the treatment. The results indicate that itis difficult to eradicate the minimal residual disease in themajority of patients with the conventional doses andcourses of alpha-interferon used at present in our coun-try.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
1995年第1期20-22,共3页
Chinese Journal of Hematology
基金
国家自然科学基金
关键词
白血病
髓细胞性
慢性
逆转录
聚合酶链反应
Leukemia, myelogenous, chronicPolymerase chain reaction bcr/ablmRNA α-interferon
