摘要
应用骨髓长期培养（ＬＴＢＭＣ）法研究了再生障碍性贫血（再障）患者血浆的巨核细胞集落刺激活性（ＭＫ－ＣＳＡ）。分别用再障血浆、正常ＡＢ混合血浆和特发性血小板减少性紫癜（ＩＴＰ）患者血浆作为刺激正常骨髓巨核细胞生长的活性来源。结果显示三组培养贴壁层形成情况类似；再障组悬浮液中累积细胞数（３．１９±０．５４）×１０￣７高于对照组（２．４８±０．６０）×１０￣７，与ＩＴＰ组（２．６４±０．６４）×１０￣７比较，统计学差异不显著；再障组悬浮液中巨核系祖细胞（ＣＦＵ－ＭＫ）维持时间长。直接对正常骨髓细胞作半固体培养，发现再障组形成的巨核细胞集落数（９５．４±３５．２／２×１０￣５个细胞）高于对照组（４３．５±２０．６／２×１０￣５个细胞）。结果表明再障血浆具有较高ＭＫ－ＣＳＡ，推测再障患者可能存在着原发性ＣＦＵ－ＭＫ缺陷，血浆中ＭＫ－ＣＳＡ升高可能是机体的一种反馈性调节。
y using long-term bone marrow culture(LTBMC )system,the megakaryocyte-colony stimulating activi-ties(MK-CSA) of the plasma from aplastic anemia(AA) patients on normal bone marrow cells were as-sayed and compared with those from patients with idio-pathic thrombocytopenic purpura(ITP)and normalcontrols(NC).The results showed that thestromalcell layer(SL) formations were similar in the threegroups,the number of nucleated cells in suspension ingroup AA(3.19 ±0.54)× 10￣7 was significantly higherthan that in control group(2.48 ±0.60)×10￣7,and theCFU-MK cultured with plasma from AA group sus-tained longer than that of the other two groups,In se-mi-solid culture of normal bone marrow cells.thenumber of MK colonies with AA plasma was also morethan that with control plasmas.
出处
《中华血液学杂志》
CSCD
北大核心
1995年第8期408-410,共3页
Chinese Journal of Hematology
关键词
再生障碍性贫血
巨核细胞
集落刺激活性
血小板
Aplastic anemia Megakaryocyte Colony stimulating activity Long-term bone marrow culture
