摘要
用聚合酶链反应快速扩增出临床分离的沙眼衣原体主要外膜蛋白基因第四可变区DNA,建立起含此DNA片段的重组质粒克隆,对重组片段序列进行了测定。从分子水平证实了沙眼衣原体母婴垂直传播。
The MOMP gene VS4 DNA of CAlanydta tracàomatts isolated from clinical samples were amplified by PCR.The recombinant plasmid containing the PCR DNA fragments were construeted.The sequences of the recombinantdsDNA were determined by Sanger's dideoxy chain termination method.Maternal-infant vertical transmission wasproved by the identical sequences of the MOMP gene of the isolates from cervix of the mother and the nasopharynxof her baby.
作者
沈犁
吴仕孝
苏国富
叶棋浓
Shen Li;Wu Shixiao;Su Guofu(Children's Hospitai,Chongqing university of Medic al Aciences,Chongqing 630014)
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
1995年第1期14-17,共4页
Chinese Journal of Microbiology and Immunology
基金
国家自然科学基金资助
关键词
沙眼衣原体
外膜蛋白基因
聚合酶链反应
Chlamydsua trachomatis
Major outer membrane protein
PCR
Sequencing
Vertical transmission
