摘要
将克隆到的中国狂犬病毒疫苗株（５ａＧ）的糖蛋白基因重组到痘苗病毒ＴＫ区，并在痘苗病毒Ｐ１１启动子的控制下，构建了狂犬－痘苗重组病毒（ＶＶａＧ）。经间接免疫荧光和Ｗｅｓｔｅｒｎ免疫印染证明，重组病毒ＶＶａＧ能良好地表达狂犬病毒糖蛋白，其分子量约为６６００。用ＶＶａＧ免疫小鼠，７ｄ便可诱生较高的狂犬病毒中和抗体，２１ｄ达４１６９，并能１００％保护狂犬病毒本毒株和国际标准攻击毒（ＣＶＳ）的致死量攻击。
A vaccinia recombinant containing the glycoprotein (G) gene of Chinese Rabies Strain (5aG), which was controlled under the vaccinia virus promoter p11, was constructed by inserting the G gene into the TK region of vaccinia virus.Through indirect immunofluorescence and western blot assays, it was shown that the recombinant VVaG can successfully express the rabies virus glycoprotein, and its molecular weight is was shown that the recombinant VVaG can induce rabies virus neutralizing antibodies (VNA) in all immunized mice and protect them against lethal challege of rabies virus aG and CVS strain intracerebally,and the highest titer of rabies VNA Can reach to 4169 on the 21th day post immunization.
出处
《中华实验和临床病毒学杂志》
CSCD
1995年第3期195-197,共3页
Chinese Journal of Experimental and Clinical Virology
