摘要
目的:研究树突状细胞(DC)在体外对恶性肿瘤凋亡细胞溶解抗原交叉提呈的可能性及由此诱导产生的抗瘤效应.方法:用As2O3体外诱导急性早幼粒白血病(APL)细胞凋亡.取患者完全缓解期(CR)外周血单个核细胞(PBMNC)体外诱导DC,体外进行APL凋亡细胞溶解抗原(ALA)负载,并设APL细胞溶解抗原(CLA)负载对照,ALADC激自体T细胞,流式细胞术分析DC,T细胞免疫表型.51Cr释放法测定T细胞对APL细胞杀伤活性.结果:APL细胞经2μmol/LAs2O3体外诱导5d,凋亡率为(86±4)%;自体T细胞被DC激化前,CD2+,CD3+,CD8+T细胞百分率为(27±5)%;被ALADC激活后,CD2+,CD3+,CD8+T细胞百分率上升至(50±12)%,对照组下降至(22±4)%.细胞毒性实验示ALADC激活的自体T细胞对APL细胞的杀伤活性明显强于对照组(P<0.01).结论:APL凋亡细胞溶解抗原能被DC交叉提呈,激活CD8+CTL细胞,其诱导的抗白血病效应强于非凋亡细胞溶解抗原致敏的DC.
AIM: To investigate the possibility of dendritic cells (DCs) cross presenting apoptotic leukemic cells lysate antigen and inducing anti-leukemic immune activity. METHODS: The apoptosis of acute promyelocytic leukemia (APL) cells was induced by arsenic trioxied. DCs derived from peripheral blood mononuclear cells (PBMNC) of patients in complete remission (CR) were pulsed with apoptotic APL cells lysate antigen (ALA) and compared with those pulsed with APL cells lysate antigen (CLA). The phonetypes of T cells and DCs were tested by flow cytometry and the cytotoxicity was tested by 51Cr release assay. RESULTS: The flow cytometry analysis showed that the apoptotic indexes were respectively (86±4)% for the APL cells induced by 2 μmol/L arsenic trioxied for five days in vitro, (27±5)% for the auto-T cells expressing CD2, CD3 and CD8 before cocultured with DCs, (50±12)% after cocultivated with ALA-DCs and (22±4)% after cocultivated with CLA-DCs. T cells activated by ALA-DCs had greater cytotoxicity against APL cells than those activated by CLA-DCs. CONCLUSION: DCs can effectively cross present apoptotic APL cells lysate antigen in vitro and activate specific CD8+CTL. These cells have greater anti-leukemic effects than those activated by CLA-DCs.
出处
《第四军医大学学报》
北大核心
2005年第14期1282-1284,共3页
Journal of the Fourth Military Medical University
