摘要
目的研究超抗原SPE对SLE患者外周血T淋巴细胞增殖及凋亡的作用。方法用SPE刺激SLE患者外周血T淋巴细胞,用流式细胞仪观察T淋巴细胞增殖、细胞表型以及凋亡情况,ELISA法检测IL2产生。结果SPE有促进SLE患者外周血T淋巴细胞增殖的作用,增殖细胞主要为CD4+T淋巴细胞,同时产生大量的IL2;再次用SPE刺激,可诱导T淋巴细胞凋亡。结论链球菌感染后,可能通过SPE诱导T淋巴细胞激活、随后出现细胞凋亡的途径而导致SLE病情活动加重。
Objective To study the effect of proliferation and apoptosis of superantigen SPE in SLE.Methods To use SPE to stimulate T lymphocyte of SLE peripheral blood;To observe proliferation, cell phenotype and apoptosis on T lymphocyte with flow cytometry;to examine IL-2 production with ELISA.Results SPE have improved on proliferation of T lymphocyte of SLE peripheral blood,specially CD4^+ cell,and producing a large of IL-2.Again with SPE stimulation,induced T lymphocyte apoptosis.Conclusion After streptococcus infected,it induced T lymphocyte apoptosis activation through SPE and then appeared lymphocyte apoptosis to lead to aggravate the state of illness activation.
出处
《中国皮肤性病学杂志》
CAS
北大核心
2005年第7期395-397,共3页
The Chinese Journal of Dermatovenereology
基金
云南省卫生厅科学研究基金(99Q028)
