摘要
背景:隔药灸治疗溃疡性结肠炎具有良好的干预效果,是否与溃疡性结肠炎发病过程中中性粒细胞的凋亡有关?目的:观察溃疡性结肠炎大鼠中性粒细胞凋亡情况及隔药灸的调节作用。设计:动物实验观察。单位:上海市针灸经络研究所。材料:实验于2002-08/2003-06完成。选择雄性清洁级SD大鼠30只,体质量(140±20)g,由上海中医药大学实验动物中心提供。按随机数字表法分为两组,造模组20只,正常对照组10只。方法:采用免疫学方法建立大鼠溃疡性结肠炎模型。随机抽取模型大鼠4只与正常组大鼠2只剖取远端结肠作组织病理学观察,在确定模型制备成功后,将剩余模型大鼠分为模型组和隔药灸组,每组8只,8只正常大鼠作为对照组。隔药灸组在双天枢及气海穴施以隔药灸,药饼以附子、肉桂等配制,艾炷约90mg置于药饼上施灸,1次/d,共灸10次。治疗结束后3组大鼠同时处死,分离外周血单个核细胞进行培养,取以上各组按1∶50稀释的培养上清液,以等体积RPMI-1640培养液作为空白对照组。分别与正常大鼠外周血中性粒细胞共同孵育,应用流式细胞术检测中性粒细胞的凋亡率。主要观察指标:各组大鼠外周血中性粒细胞凋亡率比较。结果:纳入大鼠造模组20只,正常对照组10只。造模组4只大鼠与正常组2只大鼠处死作组织病理学观察,进入结果分析造模组和正常对照组分别为16和8只。①溃疡性结肠炎大鼠中性粒细胞凋亡率显著低于空白对照组[(16.34±2.80)%,(52.33±9.94)%,(q=-35.99,P<0.01)],而正常对照组[(48.79±11.30)%]与空白对照组之间差异无显著性意义(P>0.05)。②经治疗后隔药灸组中性粒细胞凋亡率[(36.03±8.31)%]显著高于溃疡性结肠炎模型组(q=19.69,P<0.01),但仍低于正常对照组(q=-16.30,P<0.05)。结论:调节和/或减轻溃疡性结肠炎中性粒细胞凋亡被抑制的状态,可能是隔药灸治疗溃疡性结肠炎的主要机制之一。
BACKGROUND: Treatment of ulcerative colitis (UC) with herbs-partition moxibustion is of good intervenient effect, whether the effect is related to neutrophil apoptosis in the pathogenic process of UC*9芽 OBJECTIVE: To investigate the changes of neutrophil apoptosis rate of rats with UC and the regulative effect of medicinal cake moxibustion. DESIGN: A randomized controlled trial based on SD rats. SETTING: Shanghai Research Institute of Acupuncture and Meridian. MATERIALS: The experiment was completed from August 2002 to June 2003. A total of 30 healthy male SD rats of clean grade, weighing (140±20) g, provided by Experimental Animal Center of Shanghai University of Traditional Chinese Medicine, were at random divided into 2 groups: model building group (n=20) and normal control group (n=10). METHODS: Rat UC models were set up with immunological method. After the models were successively set up, four model rats and two normal rats were randomly selected for pathological observation of distal colon tissue. The rest models were divided as model group and herbs-partition moxibustion group with 8 rats for each, and the rest 8 normal rats were taken as control group. The rats of herbs-partition moxibustion group were given herbs-partition moxibustion at tian shu (ST-25) of both sides and qi hai (CV-6) points; the herbs cones was made from Radix Aeoniti Praeparata, Cortex Cinnamoni, and so on. About 90 mg moxa cone was put on the herbs cones for moxibustion, once a day, for 10 times. After treatment, all rats of the three group were executed, then the peripheral monocytes were isolated for cell culture. A 1:50 diluted supernatants of each group were taken, and the same volume of RPMI-1640 culture fluid was used as blank control group. They were respectively incubated together with neutrophils of peripheral blood of normal rats. The neutrophil apoptosis rates were measured by using flow cytometry. MAIN OUTCOME MEASURES: Comparison of neutrophil apoptosis rates of rats in each group. RESULTS: Totally 20 rats in model group and 10 in normal control group were accepted. Then 4 rats in model group and 2 in normal group were sacrificed and observed with tissue pathology, and 16 and 8 rats entered the final analysis in model and normal control groups respectivelly. ① The neutrophil apoptosis rate of UC rats was significantly lower that that of rats in blank control group [(16.34±2.80) %, (52.33±9.94) %, q= -35.99, P < 0.01]; but there was no significant difference between normal control group [(48.79±11.33) %] and blank control group, (P>0.05). ② After treatment the neutrophil apoptosis rate of herbs-partition moxibustion group [(36.03±8.31) %] was significantly higher than that of UC model group (q=19.69, P < 0.01), but it was still lower than that of normal control group (q= -16.30, P < 0.05). CONCLUSION: One of the main mechanism for herbs-partition moxibustion to treat UC might be regulating and/or reducing the inhibited state of neutrophil apoptosis in UC.
出处
《中国临床康复》
CSCD
北大核心
2005年第23期236-238,F003,共4页
Chinese Journal of Clinical Rehabilitation
基金
国家自然科学基金资助项目 (30200368)
上海市重点学科建设项目(T0302)~~
