摘要
背景:进一步验证辛夷挥发油所具有抗非特异性炎症的作用,为此实验的起因。目的:探讨辛夷挥发油对大鼠佐剂性关节炎的作用。设计:分层抽样,随机分组对照,量效分析。单位:成都中医药大学药学院药理教研室。材料:实验于1999-02/04在成都中医药大学药理实验室完成。选用Wistar大鼠120只,按体质量分层后随机分组。方法:Wistar大鼠分成4组:模型对照组,辛夷挥发油0.28mL/kg及0.14mL/kg组,地塞米松25mg/kg组。各组动物均在足跖部皮内注射完全弗氏佐剂(0.1mL/只)致敏,以诱发多发关节炎。依给药方案不同,实验分3批次进行,各个批次每组大鼠10只。第1批次在致敏前3d开始给动物灌药,连续给药3d,观测药物对佐剂引起的急性非特异性炎症的作用;第2批次在致敏后第8天开始给动物灌药,连续给药7d,观测药物对继发病变的预防作用;第3批次在致敏后第19天开始给动物灌药,连续给药7d,观测药物对继发病变的治疗作用,。测量大鼠足肿胀程度及距小腿(踝)关节组织中前列腺素E2含量。主要观察指标:①辛夷挥发油对佐剂引起的急性非特异性炎症的作用。②对佐剂性关节炎的预防作用。③对佐剂性关节炎的治疗作用。结果:①辛夷挥发油能极显著抑制原发性足肿胀。②预防给药时辛夷挥发油0.28mL/kg,0.14mL/kg组均能极显著地抑制继发性关节肿胀[(0.231±0.065)mL,(0.206±0.082)mL],减少肿胀组织中前列腺素E2含量[(0.1332±0.0572),(0.1385±0.0426)A278nm]。③治疗性给药时,辛夷挥发油0.14mL/kg组对继发性关节炎性肿胀无明显作用,但能显著减少肿胀组织中前列腺素E2含量;辛夷挥发油0.28mL/kg组对上述参数有极显著或显著作用。结论:辛夷挥发油对完全弗氏佐剂引起的原发性足肿胀和继发性关节炎都有抑制作用,预防作用优于治疗作用,治疗作用时大剂量组效果较好。
BACKGROUND:The aim of present study is to demonstrate that volatile oil of flos magnoliae biondii pamp(VOMbp) possess anti-immunology inflammatory property. OBJECTIVE:To explore the effect of VOMbp on adjuvant arthritis in rats. DESIGN:Grade sampling,randomized controlled quantitative analysis. SETTING:Teaching and Researching Department of Pharmacology,Chengdu University of Traditional Chinese Medicine. MATERIALS:This study was carried out at the Teaching and Researching Department of Pharmacology,Chengdu University of Traditional Chinese Medicine from December to April in 1999. Totally 120 Wistar rats were involved. METHODS:Wistar rats were randomly divided into 4 groups:model control group, 0.28 mL/kg and 0.14 mL/kg VOMbp group,and 25 mg/kg dex-amethasone group. Allergy were induced in all rats through intra-cutaneous injection of Freund's adjuvant(0.1 mL/rat)at metatarsus so as to establish multiple arthritis model in rat. According to different dosage,the experiments were carried out in three batches with 10 rats in each group. Rats in the first batch were given VOMbp three days before inducing allergy for consecutive 3 days,in order to observe the influence of VOMbp on adjuvant induced acute non-specific inflammatory;Rats in the second batch were given VOMbp on the 8th day sensitization allergy for consecutive 7days so as to explore the protective effect on secondary pathological changes;rats in the third batch were administratedon the 19th day after sensitization for consecutive 7 days for study in therapeutic effect on secondary pathological changes.Meanwhile foot tumefaction and the level of prostaglandin E2 in ankle arthritis were observed. MAIN OUTCOME MEASURES:① Effect of VOMbp on the adjuvant induced acute non-specific inflammatory. ② Preventive effect on adjuvant arthritis. ③ Therapeutic effect on adjuvant arthritis. RESULTS:① VOMbp could significantly suppress the primary foot tume faction.②Moreover VOMbp of preventive dosage could most significantly suppress the secondary arthritis tumefaction[(0.231±0.065)mL of 0.28 mL/kg group and (0.206±0.082)mL of 0.14 mL/kg group],accompanied by PG-E2 decrement in tumefaction tissues[(0.133 2±0.057 2),(0.138 5 ±0.042 6)OD278nm correspondingly].③ No obvious effect of VOMbp of therapeutic dosage on secondary inflammatory tumefatcion of arthritis could be observed in 0.14 mL/kg group,but obvious decrement of prostaglandin E2 could be observed in edema tissue;While 0.28 mL/kg VOMbp exerted most significant or significant influence on above parameters. CONCLUSION:VOMbp possess inhibiting effect on Freund's adjuvant induced primary foot tumefaction and secondary arthritis,with the preventive effect better than therapeutic effects.The therapeutic effect was proved better in higher dosage group.
出处
《中国临床康复》
CAS
CSCD
北大核心
2005年第23期210-211,共2页
Chinese Journal of Clinical Rehabilitation
