摘要
目的:采用D-半乳糖致亚急性衰老大鼠模型,观察天年饮对血脂及其代谢产物的调节效应。方法:实验于2003-04/07在承德医学院中心实验室完成。选择成年雄性SD大鼠40只,随机分为正常组、衰老模型组、天年饮用药组和阴性对照组,每组10只。衰老模型组、天年饮用药组和阴性对照组均采用D-半乳糖200mg/(kg·d)连续腹腔注射40d制作亚急性衰老大鼠模型。衰老模型组大鼠于造模成功后不再作任何处理;天年饮用药组大鼠于造模成功后用天年饮(由何首乌15g,怀牛膝15g,肉苁蓉10g,淫羊藿10g,丹参25g,茯苓15g组成,每副含生药90g,按传统方法加水煎成溶液)8.1g/kg灌胃给药,1次/d,共30d;阴性对照组大鼠于造模成功后每天灌胃同等剂量的生理盐水,时间同天年饮用药组。正常组大鼠自由摄食、饮水,未加任何干预措施。所有大鼠经内眦于眶后静脉丛采血约2mL,检测血清总胆固醇、三酰甘油及高密度脂蛋白胆固醇的含量。结果:40只大鼠全部进入结果分析。①衰老模型组血清中总胆固醇、三酰甘油的含量明显高于正常组[(2.68±0.39),(1.19±0.45)mmol/L;[0.84±0.29),(0.44±0.23)mmol/L,(t=-3.470,P<0.01,t=-2.834,P<0.05)]。②天年饮用药组血清中总胆固醇、三酰甘油的含量明显低于衰老模型组[(1.91±0.53),(2.68±0.39)mmol/L;(0.44±0.12),(0.84±0.29)mmol/L,(t=3.108,P<0.01,t=3.256,P<0.05)]。③衰老模型组血清中高密度脂蛋白胆固醇的含量低于正常组[(1.03±0.17),(1.46±0.38)mmol/L,(t=2.709,P<0.05)]。④天年饮用药组血清中高密度脂蛋白的含量明显高于衰老模型组[(1.45±0.44),(1.03±0.17)mmol/L,(t=-2.371,P<0.05)]。结论:天年饮可降低衰老大鼠血清总胆固醇、三酰甘油的水平及提高高密度脂蛋白胆固醇的含量,说明天年饮可有效调节衰老大鼠血脂代谢紊乱。
AIM: To observe the effects of Tiannian Yin in adjusting blood lipid and its metabolic products in D-galactose induced sub-acute aging rats. METHODS: The experiment was carried out in the central laboratory of Chengde Medical College from April to July 2004. Forty adult male SD rats were randomly divided into normal group (n=10), aging model group (n=10), Tiannian Yin medication group (n=10) and negative control group (n=10). Rats in the aging model group, Tiannian Yin medication group and negative control group were made into sub-acute aging models by intraperitoneal injection of D-galactose (200 mg/kg per day) for 40 continuous days; After successful establishment of models, no treatment was given to the rats in the aging model group, rats in the Tiannian Yin medication group were treated with gastric perfusion of 8.1 g/kg Tiannian Yin (consisted of tuber fleeceflower root 15 g, achyranthes root 15 g, desertliving cistanche 10 g, epimedium 10 g, danshen 25 g and Tuckahoe 15 g; Each dose containing 90 g crude drug, and decocted into solution with water with traditional method), once a day for 30 days, and those in the negative control group were intragastrically perfused with saline of the same volume, the time was the same as that in the Tiannian Yin medication group; Rats in the normal group could eat and drink freely, and received not any intervention. Blood sample (about 2 mL) was drawn from the venous plexus of posterior orbit throughmedial canthus in all the rats, and then the serum contents of total cholesterol, triglyceride and high density lipoprotein cholesterol were detected. RESULTS: All the 40 rats were involved in the analysis of results. ① The serum contents of total cholesterol and triglyceride contents were obviously higher in the aging model group than in the normal group [(2.68±0.39), (1.19±0.45) mmol/L; (0.84±0.29), (0.44±0.23), t =-3.470, P < 0.01, t= -2.834, P < 0.05]. ② The serum contents of total cholesterol and triglyceride contents were obviously lower in the Tiannian Yin medication group than in the aging model group [(1.91±0.53), (2.68±0.39) mmol/L; (0.44±0.12) , (0.84±0.29) mmol/L, t=3.108, P < 0.01, t= 3.256, P < 0.05]. ③ The content of high density lipoprotein cholesterol in serum was lower in the aging model group than in the normal group [(1.03±0.17), (1.46±0.38) mmol/L, t=2.709, P < 0.05]. ④ The content of high density lipoprotein cholesterol in serum was obviously higher in the Tiannian Yin medication group than in the aging model group [(1.45 ±0.44), (1.03±0.17) mmol/L,t=-2.371,P < 0.05]. CONCLUSION: Tiannian Yin can decrease the levels of total cholesterol and triglyceride and increase the content of high density lipoprotein cholesterol in serum of aging rats, it is indicated that Tiannian Yin can effectively adjust the metabolic disorders of blood lipid in aging rats.
出处
《中国临床康复》
CSCD
北大核心
2005年第23期136-137,共2页
Chinese Journal of Clinical Rehabilitation
