摘要
目的:观察针刺和饮食结构调整对实验性肥胖大鼠的影响。方法:实验于2004-05/08在上海针灸经络研究所完成,选用SD雄性大鼠,随机分为2组。肥胖模型组造模成功后将其48只分成6组,针刺+普食组、针刺对照+普食组、针刺+高脂组、针刺对照+高脂组、肥胖模型普食组、肥胖模型高脂组,各组8只。正常组随机取8只。针刺治疗取一侧足三里、内庭,20min/次,隔日1次,连续15次,左右侧轮流取穴。针刺对照组将大鼠放置于30cm高的平台20min,不进行针刺治疗,隔日1次,连续15次。将普通饲料喂养的雄性大鼠作为正常组。肥胖模型高脂组喂高脂饮食,即每100g普通饲料中加入奶粉10g,猪油10g,鸡蛋1只,浓缩鱼肝油0.1mL(每粒含维生素A1万单位,维生素D21000单位),黄豆芽250g。干预30d后,观察两组体质量、体长、Lee's指数(√3体质量×103/体长)、饮食量、饮水量的变化。结果:模型组48只实验过程中针刺+普食组和肥胖模型高脂组各脱失1只,加正常组8只,进入结果分析54只。①针刺+普食组体质量、Lee's指数与正常组比较差异无显著性意义(P>0.05),其他各组与正常组比较差异有显著性意义(P<0.01)。②针刺+普食组干预后体质量、Lee's指数均低于肥胖模型高脂组[(456.79±25.76)g,(301.99±7.06);(493.49±26.92)g,(313.32±4.70),P<0.05],其他各组上述两项指标与肥胖模型高脂组差异不明显。③针刺+普食组干预30d后体质量明显低于针刺+高脂组[(456.80±25.76),(486.85±18.47)g,P=0.021]。④针刺+普食组与肥胖模型普食组比较干预后,体质量无显著差别。结论:针刺结合饮食结构调整对肥胖大鼠的作用较单一针刺和单一饮食结构调整显著,各组总饮食量比较,也说明针刺与饮食结构控制共同作用抑制食欲效果较好。
AIM: To observe the influence of acupuncture and adjustment of dietary structure on experimental obese rats. METHODS: The experiment was carried out in the Shanghai Institute of Acupuncture and Meridian between May and August 2004. Male SD rats were randomly divided into two groups. After model establishment, 48 rats in the obese model group were divided into 6 groups with 8 rats in each group: acupunture+general feed group, acupuncture control+ general feed group, acupunture+high fat diet group, acupuncture control+ high fat diet group, obese model general feed group and obese model high fat diet. Eight normal rats were randomly selected as normal group.Acupuncture treatment at unilateral acupoints of Zuzanli and Neiting was given 20 minutes for each time, once every other day for 15 times continuously on the left and right by turn. Rats in the acupuncture control groups were placed on the plat of 30 cm high for 20 minutes without acupuncture treatment, once every other day for 15 times continuously. Male rats fed with general diet were taken as normal group; Obese model high fat diet group were fed with high fat diet, that was, 100 g general feed was added with 10 g milk powder, 10 g lard, an egg, concentrated cod-liver oil (each granule contained vitamin A of 10 000 units and vitamin D2 of 1 000 units), and 250 g soybean sprout. Thirty days after intervention, the changes of body mass, body length, Lee's index (3√body mass×103/body length), dietary amount and amount of drinking water were observed in both groups. RESULTS: Among the 48 rats in the model groups, 1 in the acupuncture+general feed group and obese model high fat feed group respectively died during the experiment, together with 8 rats in the normal group, totally 54 rats were involved in the analysis of results. ① Body mass and Lee's index in the control group had insignificant difference as compared with that in the acupuncture+general feed group (P > 0.05), but significantly different from those in the other groups (P < 0.01). ② After intervention, the body mass and Lee's index in the acupuncture+general feed group were lower than those in the obese model high fat feed group [(456.79±25.76) g, (301.99±7.06); (493.49±26.92) g, (13.32±4.70), P< 0.05], and the two indexes were not obvious different between the obese model high fat feed group and other groups. ③ The body mass after intervention was significantly lower in the acupuncture+general feed group than in the acupuncture+ high fat feed group [(456.80±25.76), (486.85±18.47) g, P=0.021]. ④ The body mass after intervention had no significant difference between the acupuncture+general feed group and obese model general feed group. CONCLUSION: Acupuncture combined with adjustment of dietary structure has significant effect on obese rats as compared with simple acupuncture or adjustment of dietary structure. The comparison of dietary amount among the groups also indicates that the synergistic action of acupuncture and control of dietary structure has better effect in restricting appetite.
出处
《中国临床康复》
CSCD
北大核心
2005年第23期31-33,共3页
Chinese Journal of Clinical Rehabilitation
基金
国家中医药管理局针灸学重点学科资助
上海市教委针灸学重点学科资助~~
