摘要
建立了食品中曲酸(KA)的离子对高效液相色谱法。采用0.01mol/L磷酸氢二钠-甲醇(95:5,V/V)为流动相,添加1.0mmol/L四丁基溴化胺(TBA),检测波长226nm,样品用40%甲醇超声提取,曲酸在Nova-PakC18柱上获得了良好分离。该方法的检出限为0.032mg/L,回收率为95.7%～103.2%,相对标准偏差为1.16%～2.01%。结果表明,方法的检出限为0.032mg/L,回收率为95.7%～103.2%,相对标准偏差为1.16%～2.01%。该法准确、快速、简便,应用于食品中曲酸的测定,结果满意。
In this paper a new and an efficient method for analyzing kojic acid(KA) in food by ion-pair high performance liquidchromatography was developed.Nova-PakC18 column was used and mobile phase was 0.01mol/L Na2HPO4-methanol(95:5,V/V)with addition of 1.0mmol/L tetrabutylammonium bromide(TBA).The wavelength 226nm was selected for detection.Sampleswere extracted with 40%methanol by ultrasonic method. Under the optinum conditions, the detection limit was 0.032mg/L. Therecoveries were 95.7%～103.2% and the relative standard deviations were 1.16%～2.01%. This method was accurate, quick-acquired and convenient.It is used in the determination of kojic acid with satisfactory result.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2005年第7期205-207,共3页
Food Science
基金
湖南省自然科学基金资助项目(03JJY5001)
湖南省教育厅科研资助项目(03C028)
关键词
高效液相色谱法
曲酸
食品
四丁基溴化胺
high performance liquid chromatography
kojic acid
food
tetrabutylammonium bromide
