摘要
本实验采用^（１２５）Ｉ标记单抗ＨＩ_（３０）的Ｆ（ａｂ＇）_２片段，^（１３１）Ｉ标记脂质体膜的方法制备免疫脂质体，并观察其在Ｓｅｐｈａｃｒｙｌ－３００层析柱上的分离结果。证明超声法制备的脂质体粒度不均一，且粒度小的脂质体具有较高的单抗结合率。初步结果还显示，单抗Ｆ（ａｂ＇）_２片段与脂质体的偶联将明显增加免疫脂质体的粒度。
The immunoliposomes were prepared by conjugating the F(ab')2 of monoclonal antibodies HI30(CD45) labelled by 125I with the liposomes labelled by 131I,and then were separated by a sephacryl S-300 column. The chromatography showed that the liposomes prepared by ultrasonic method were not homogeneous, and the liposomes of smaller granule size had a higher conjugate ratio. It was also found that the fragment F(ab')2 of McAb conjugated to liposomes could enlarge their granule size.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
1995年第6期424-427,共4页
Acta Academiae Medicinae Sinicae
基金
国家自然科学基金
关键词
免疫脂质体
粒度
单抗结合率
immunoliposomes
granule size
McAb conjugate ratio
