摘要
目的:应用基因芯片技术筛选人膀胱移行细胞癌复发相关基因。方法:使用人肿瘤基因表达谱芯片检测11例膀胱移行细胞癌患者的正常膀胱粘膜组织、原发膀胱癌组织及复发膀胱癌组织的基因表达谱,筛选出在原发膀胱癌组织及复发膀胱癌组织中差异表达的基因,并用半定量RT-PCR对部分差异表达基因进行验证。结果:以正常膀胱粘膜组织为对照,11例膀胱肿瘤组织中有87个基因表达明显下调,102个基因表达明显上调,其中有15个基因的表达在原发膀胱癌组织及复发膀胱癌组织呈相反变化。结论:膀胱肿瘤与正常膀胱粘膜组织之间存在大量差异表达的基因,说明膀胱肿瘤的发生与发展是多种肿瘤相关基因表达失常或肿瘤抑制基因失活所致;在原发膀胱癌组织及复发膀胱癌组织中呈反向变化的15个基因可能与膀胱癌的复发有关。
Objective: To screen and identify the differential-expressed genes in recurrent human transitional cell carcinoma (TCC) of the bladder. Methods: The technique of DNA chip was used to detect the gene expression spectrum of primary TCC tissues and recurrent TCC tissues from 11 human transitional cell carcinoma, and then make a further confirmation by using semi-quantity RT-PCR. Results: In contrast to normal mucous membrane tissues of the bladder, there were 189 differencially expressed genes in which 102 genes were obviously up-regulated and 87 genes down-regulated in human TCC tissues. There were 15 genes being expressed in the opposite direction between primary and recurrent TCC tissues as identified by semi-quantitative RT-PCR. Conclusions: There are many genes differentially expressed between the TCC tissues and the normal mucous membrane tissues of the bladder. The 15 genes, whose expression are opposite in primary and recurrent TCC tissues, probably result in the recurrence of human TCC.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2005年第13期759-762,共4页
Chinese Journal of Clinical Oncology
