摘要
目的建立免疫印迹法(WB)检测抗毒蕈碱受体3(M3受体)抗体的方法,探讨该抗体对干燥综合征(SS)诊断的意义。方法建立WB测定抗M3受体抗体的方法,检测80例SS患者、80例其他风湿性疾病及40名正常对照者血清中的抗M3受体抗体,并分析该抗体与SS的临床及实验室指标的关系。结果抗M3受体抗体在80例SS患者中的阳性率为83.8%(67/80),而在系统性红斑狼疮(SLE)及正常人中分别为5.0%(2/40)和2.5%(1/40),类风湿关节炎(RA)中无1例阳性。抗M3受体抗体对SS的敏感性和特异性分别为83.8%和97.5%,阳性预测值为95.7%,阴性预测值为90.0%。抗M3受体抗体在抗SSA抗体、抗SSB抗体、琢-胞衬蛋白抗体阴性的SS患者血清中的阳性率分别为80.0%、82.5%及81.2%。该抗体在抗SSA抗体、抗SSB抗体及琢-胞衬蛋白抗体阳性的SS患者血清中的阳性率分别为87.5%、88.2%及85.4%。抗M3受体抗体阳性的SS患者与该抗体阴性的SS患者在口干、眼干、脏器受累的发生率,IgG、IgA、血沉(ESR)升高及抗核抗体(ANA)、类风湿因子(RF)阳性率方面无统计学意义。结论利用sf9细胞表达的重组M3受体蛋白可以检测SS患者血清中的抗M3受体抗体。该抗体是一种对SS诊断较为特异的标记性抗体之一。
Objective To detect anti-M3 autoantibodies in Sjgren′s syndrome (SS) patients and to explore its association with clinical manifestation. Methods Anti-M3 autoantibodies were tested in 80 patients with SS, 80 with other rheumatic diseases and 40 normal controls using Western blot. The correlation between anti-M3 autoantibodies and clinical data of SS was analyzed. Results {1}The positive rate of anti-M3 antibodies was 83.8% in SS, 2.5% in other rheumatic diseases and 2.5% in normal controls. {2}Its sensitivity was 83.8% and specificity was 97.5% in SS with a positive predictive rate of 95.7% and negative prediction rate of 90.0%. {3}The positive rate of anti-M3 antibodies was 80.0%, 82.5% and 81.2% in SS with SSA, SSB and ?琢-Fodrin antibodies negative group, and 87.5%, 88.2% and 85.4% respectively in above antibodies positive group 4. The incidence of organ involvement, IgG, IgA and ESR elevation was not significantly different in anti-M3 antibodies positive and negative group. There was no significant difference in dry eye and dry mouth between antibody positive and negative group. Conclusion Human recombinant M3 muscarinic receptor expressed in sf9 cells can be used to detect anti-M3 autoantibodies in the serum from SS patient. The antibody may be a new and specific serum maker for the diagnosis of SS.
出处
《中华风湿病学杂志》
CAS
CSCD
2005年第7期409-412,共4页
Chinese Journal of Rheumatology
