摘要
目的建立监测肺结核患者口服利福平后的血药浓度,为临床合理用药提供可靠依据。方法高效液相法色谱条件:NOVAPAKC18柱,(4μm,3.9mm×150mm);流动相:甲醇0.02mol·L-1醋酸钠溶液(62∶40);流速1.0ml/min,检测波长为334nm;柱温20℃。结果血清中利福平在0.5～16.0μg·ml-1范围内浓度与峰面积线性关系良好,r=0.9992,平均回收率为99.43%。30例肺结核患者一次口服利福平600mg后,28例血药浓度检测结果在0.51～7.81μg·ml-1之间,2例血药浓度小于0.5μg·ml-1。结论本法准确、灵敏,用血量少,适用于治疗药物监测,可为临床合理用药提供数据参考。
Aim To measure the concentration of rifampicin in patients with pulmonary tuberculosis in order to provide reference data for the rational medicine usage in clinic.Methods The assay was conducted on C(18) Nova-pak column(4μm,3.9mm×150mm) with the mobile phase of the mixture of CH3OH-0.02mol·L^(-1)NaAc(62∶40) at a rate of 1.0ml/min,the UV detection wavelength was at 334nm.The column temperature was 20℃.Results A good linearity was obtained from 0.5～16.0μg·ml^(-1) with a correlation coefficient of 0.999 2.The average recovery of the assay was 99.43%.Among thirty patients by given 600mg orally,twenty eight cases'serum drug concentrationrange was 0.51～7.81μg·ml^(-1) except that two cases' ones were less than 0.5μg·ml^(-1).Conclusion The method appeared to be accurate and sensitive.It can be used for monitoring rifampicin concentration in serum and provided reference data for rational drug usage in clinic.
出处
《解放军药学学报》
CAS
2005年第3期222-224,共3页
Pharmaceutical Journal of Chinese People's Liberation Army
关键词
利福平
高效液相色谱法
血药浓度
Rifampicin
HPLC
Determine RFP concentration in serum
