摘要
将胃癌单克隆抗体ＭＧｂ２与脂质体连接，分别包入１１种抗肿瘤药物和硼－１０化合物，制成９种胃癌特异性免疫脂质体和２种硼化胃癌免疫脂质体，采用个同的人胃癌裸鼠模型和个问的给药途径，综合评价其体内外疗效和生物学分布。９种免疫脂质体较好地保持了抗体的免疫活性，对胃癌细胞只有高度选择性矛伤作用。疗效均显著优于非特异脂质体和游离药物。硼化兔疫脂质体结合热中子照射后显示出导向硼中子俘获治疗效应。结果表明，胃癌免疫胎质体在体内外均具有特斤性抗肿瘤７作用，将可用于人胃癌的临床导向治疗。
ine kinds of antineoplastic agents and
2boronlo compounds were encapsulated respectivelyinto the immunoliposomes targeted with
monoclonalantibody, MGb2, against hurnan gastric cancer. Theimrnunoliposomes were
characterized in vitro and invivo with different nude mouse xenograft models anddifferent routes
of administration. The immunore ac-tivity was well preserved. The diameter was 100nm
forreversephase evaporation vesicles and 3 snm for smallunilamellar vesicles. 1 000￣10 000
molecules ofantitumor agent were entrapped and 30￣80 moleculesof MGb2 incorporated per
one liposoine. Theyshowed selective cytotoxicity to human gastric cancercell SGC-7901 but
very low toxicity to human normalembryonic lung cell SL7. Biodistnbution studies indi-cated
that both were better than non-specificliposomes and free drugs. Boronated immuno-liposomes
could carry boron-10 specifically toSGC-7901 and showed targeted boron neutron cap-ture
therapy effects after thermal neutron irradiation.The results confirmed the specific antitumor
effects ofimmunoliposomes in vitro and in vivo and demon-strated the potentiality of the
immunoliposomes in thetargeting therapy of human gastric cancer.
出处
《中华医学杂志》
CAS
CSCD
北大核心
1994年第2期83-86,T009,共5页
National Medical Journal of China
基金
国家自然科学基金
国家863计划青年基金
关键词
脂质体
抗体
单克隆
胃肿瘤
Liposoines
Antibodies, mono-clonal Stomach neoplasms Mice, nude
