摘要
新西兰白兔经角膜接种ＨＳＶ－１Ｍｃｋｒａｅ株后，在三叉神经节（ｔｒｉｇｅｍｉｎａｌｇａｎｇｌｉｏｎ，ＴＧ）内形成潜伏感染。当接种病毒后第３０～１００天时，分别摘除ＴＧ做组织细胞培养，在培养到第８天时，ＴＧ细胞内出现ＨＳＶ－１抗原阳性，第１０天时培养细胞开始呈现细胞病变（ｃｅｌｌｐａｔｈｏｌｏｇｉｃａｌｅｆｆｅｃｔｓ，ＣＰＥ），第２～３周内全部潜伏感染的ＴＧ培养细胞出现自发性感染，ＣＰＥ和ＨＳＶ－１抗原均为阳性。在体外成功地建立了潜伏感染ＴＧ活化的细胞模型，为进一步研究ＨＳＶ－１在ＴＧ细胞内的潜伏感染机制提供了一个新的途径。
AbstractLatent infection in the trigeminal ganglion(TG)was established by inoculating HSV-1 strain Mckraeon the cornea of New Zealand white rabbits.From PIday 30 to day 100,we removed the TG at intervals toestablish primary cell cultures.HSV-1 antigen waspositive in the TG culture cells on PI day 8 and CPEappeared on PI day 10. All latently infected TG cul-ture cells showed spontaneous infection,while CPEand HSV-1 antigen were positive in PI 2 to 3 weeks.We thus estabhshed a cell model in Which HSV-1 la-tent infection in TG was activated in vitro,This mayserve as a new approach to study the mechanism ofHSV-1 latent infection in TG.
出处
《中华眼科杂志》
CAS
CSCD
北大核心
1994年第1期44-46,T003,共4页
Chinese Journal of Ophthalmology
