摘要
人骨髓细胞体外长期培养基质层细胞按其核浆、形态特征分为四类：ＡＣ１最大，有数个明显核仁，胞浆呈薄纱状；ＡＣ２与巨噬细胞类似，核小深染，胞内有吞噬物；ＡＣ３最显著的特征是胞浆内广泛分布的脂质空泡；ＡＣ４最小，核大深染，胞浆少。从培养第４周至第８周，ＡＣ１及ＡＣ２分别从２１．７５％、３１．２５％上升至４０．５％、５５％；ＡＣ３及ＡＣ４分别从３１％、１５．５％降至１．５％、３％。碱性磷酸酶－抗碱性磷酸酶（ＡＰＡＡＰ）法检测细胞表面抗原，可见ＡＣ１纤维联结蛋白阳性，ＡＣ２的ＣＤ６８强阳性，ＡＣ３的纤维联结蛋白弱阳性，ＡＣ４的上两种单抗及因子ＶＩＩＩ相关活性抗原均标记阴性。本实验结果可作为一个参考值，衡量在各种实验条件下基质层细胞组成的变化。
In this study,we divided adherent cells into four classes according to their morphological characteristics (AC1, AC2, AC3, AC4). AC1 was the largest cells,containing several nucleoli and thin cytoplasm, AC2 was similar to the macrophage, with small, eccentrical,condensed nuclei and coarse granules in its cytoplasm. Oharacteristics of AC3 included marked vacuolations in its cytoplasm and different sized nuclei; AC4 was the smallest cell, with big, condensed nuclei and scanty cytoplasm, after 4 to 8 weeks of culture, AC1 increased from 21.75% to 40.5%, AC2 from 31.25% to 55%, and AC3, AC4 decreased from 31.5%, 15.5% to 1.5% , 3% , respectively.Using monoclonal antibodies and the APAAP method, AC1 was found to be fibronectin positive;AC2 was CD68 strongly positive, and AC3 was fibronectin weakly positive, AC4 was found to be fibronectin, CD68, and factor VIIIc negative.These observations were of help in the identification of changes in the composition of adherent cells in long-term human bone marrow culture under various experimental conditions.
出处
《上海医科大学学报》
CSCD
1994年第5期363-366,T022,共5页
Journal of Fudan University(Medical Science)
关键词
骨髓
基质层细胞
细胞培养
long-term bone marrow culture
stromal cells
alkaline-phosphatase/anti-alkaline-phosphatase method
