摘要
目的对广东地区汉族MS患者作肿瘤坏死因子基因(tumornecrosisfactor,TNFβ)分型,探讨TNFβ基因与广东人群中MS遗传易感性的可能关系。方法采用聚合酶链式反应限制性片段长度多态性技术对30名无亲缘关系的MS的病人和103名无血缘关系的广东籍健康汉族人作TNFβ分型。结果TNFβ各等位基因型频率在MS组和正常人组间无显著差异(P均>0.5)。结论从目前调查的例数,广东人群中MS与TNFβ基因不关联。
Objective To investigate the distribution of TNFβ genes in Guangdong population, examined the association of these genes with multiple sclerosis. Methods The normal subjects consisted of 103 unrelated healthy individuals of han nationality from the Guangdong region. 30 patients with multiple sclerosis, all from the same geographic region, TNFβ were genotyped by the PCR-RFLP method, TNFβ genotypes were assigned according to electrophoretic patterns of the nucleotide fragments produced by NcoⅠ digestion of the specific PCR products. Results There was no significant differences in allelic frequencies of TNFβ gene between the patients and normal controls. Conclusion The polymorphisms of TNFβ genes is not associated with MS in Guangdong region.
出处
《广州医药》
2005年第4期10-12,共3页
Guangzhou Medical Journal
